Recombinant Human MSLN protein, His & GST-tagged

• Cat.No.: MSLN-1538H
• Product Overview: Recombinant Human MSLN aa. (Glu400~Leu615) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
• Source: E. coli
• Species: Human
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: 54kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Glu400~Leu615
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >92%
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 5.2

Gene Information

• Gene Name: MSLN mesothelin [ Homo sapiens (human) ]
• Official Symbol: MSLN
• Synonyms: MSLN; mesothelin; MPF; SMRP; CAK1 antigen; megakaryocyte potentiating factor; pre-pro-megakaryocyte-potentiating factor; soluble MPF mesothelin related protein
• Gene ID: 10232
• mRNA Refseq: NM_001177355.2
• Protein Refseq: NP_001170826.1
• UniProt ID: Q13421