Recombinant Human MME protein, His-tagged

• Cat.No.: MME-1807H
• Product Overview: Recombinant Human MME protein(P08473)(Tyr52-Trp750), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Tyr52-Trp750
• Tag: N-His
• Form: Supplied as 0.22μm filtered solution in 50mM MES, 100mM NaCl, 1mM ZnCl2, 10% glycerol (pH 6.5).
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK (Dnp) -OH. The specific activity is > 1500 pmoles/min/ug.
• Molecular Mass: The protein has a predicted MW of 80.9 kDa. Due to glycosylation, the protein migrates to 81-110 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MME membrane metallo-endopeptidase [ Homo sapiens ]
• Official Symbol: MME
• Synonyms: MME; membrane metallo-endopeptidase; neprilysin; CALLA; CD10; enkephalinase; NEP; neutral endopeptidase; atriopeptidase; skin fibroblast elastase; neutral endopeptidase 24.11; membrane metallo-endopeptidase variant 1; membrane metallo-endopeptidase variant 2; common acute lymphocytic leukemia antigen; membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase); membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10); SFE; MGC126681; MGC126707; DKFZp686O16152
• Gene ID: 4311
• mRNA Refseq: NM_000902
• Protein Refseq: NP_000893
• MIM: 120520
• UniProt ID: P08473