Active Recombinant Mouse Mme protein, His-tagged

• Cat.No.: Mme-266M
• Product Overview: Recombinant Mouse Mme protein(NP_032630.2)(Tyr52-Trp750), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Tyr52-Trp750
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK-(Dnp)-OH.The specific activity is > 3000 pmoles/min/μg.
• Molecular Mass: The secreted recombinant mouse Mme consists of 715 amino acids and has a calculated molecular mass of 82.2 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 90-100 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Mme membrane metallo endopeptidase [ Mus musculus ]
• Official Symbol: Mme
• Synonyms: MME; membrane metallo endopeptidase; neprilysin; enkephalinase; atriopeptidase; skin fibroblast elastase; neutral endopeptidase 24.11; common acute lymphoblastic leukemia antigen; NEP; SFE; CD10; CALLA; C85356; 6030454K05Rik
• Gene ID: 17380
• mRNA Refseq: NM_008604
• Protein Refseq: NP_032630