Recombinant Human (minus BH3 domain) B-Cell CLL/Lymphoma 2, His-tagged

• Cat.No.: BCL2-4222H
• Product Overview: Bcl-2 Des BH3 domain (93-107 residues) Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 203 amino acids 1-92 and 108-218. The Bcl-2 is expressed as His-Tag fusion protein and purified by proprietary chromatographic techniques.

Specification

• Cat. No.: BCL2-4222H
• Description: BCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
• Source: Human (minus BH3 domain)
• Host: Escherichia Coli.
• Form: The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.
• Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Solubility: Suspend Bcl-2 in 100?l of 0.5M Acetic acid, over night at 4°C. Dilute 10 fold into selected buffer system. BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.
• Storage: Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Bcl-2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Protein length: 93-107;108-218 a.a.

Gene Information

• Gene Name: BCL2 B-cell CLL/lymphoma 2 [ Homo sapiens ]
• Official Symbol: BCL2
• Synonyms: Bcl-2; OTTHUMP00000163680; BCL2; B-cell CLL/lymphoma 2; B-cell lymphoma protein 2
• Gene ID: 596
• mRNA Refseq: NM_000633
• Protein Refseq: NP_000624
• MIM: 151430
• UniProt ID: P10415
• Chromosome Location: 18q21.33; 18q21.3
• Pathway: Amyotrophic lateral sclerosis (ALS); Apoptosis; Colorectal cancer; Focal adhesion; Neurotrophin signaling pathway; Pathways in cancer; Prostate cancer; Small cell lung cancer
• Function: BH3 domain binding; Protease binding; Protein Heterodimerization activity; Protein homodimerization activity; Transcription activator activity; Transcription factor binding