Recombinant Human B-cell CLL/lymphoma 2, His-tagged

• Cat.No.: BCL2-5361H
• Product Overview: Recombinant human BCL2was expressed in E. coli.

Specification

• Cat. No.: BCL2-5361H
• Description: Recombinant humanBCL2 with His Tag, excluding the BH3 domain, which corresponds to amino acids93-107. This is a single, non-glycosylated polypeptide chain containing 203amino acid residues consisting of amino acids 1-92 and 108-218.
• Source: E. coli
• Species: Human
• Form: Lyophilized from asterile filtered solution containing 10 mM Tris-HCI, pH 8.0+1 mM EDTA+250 mMNaCI.
• Purity: >95% asdetermined by RP-HPLC and SDS-PAGE analyses
• Endotoxin Level: <0.1 ng/ug ofBCL2
• Reconstitution: Centrifuge vialprior to opening. Suspend BCL2 in 100 ul of 0.5 M Acetic acid, over night at2-4°C. Dilute 10 fold into selected buffer system. Because BCL2 has atendency to form intramolecular disulfide bond, 5 mM DTT is recommended inassay buffer. When running SDS-PAGE gel, 10 mM DTT is recommended.
• Applications: Western Blot; BindingAssay
• Storage: Store lyophilizedprotein at -20°C to -80°C. Reconstituted protein is stable for 1 week at 2-4°C.For long term storage, aliquot and store at -20°C to -80°C with a carrierprotein (0.1%HSA or BSA) as a stabilizer. Please note that the addition ofany carrier protein into this product may produce unwanted endotoxin. Thisdepends upon the particular application employed. Avoid repeated freeze-thawcycles.
• OfficialSymbol: BCL2

Gene Information

• Gene Name: BCL2 B-cell CLL/lymphoma 2 [ Homosapiens ]
• Synonyms: BCL2; B-cellCLL/lymphoma 2; Bcl-2; PPP1R50; apoptosis regulator Bcl-2; proteinphosphatase 1, regulatory subunit 50
• Gene ID: 596
• mRNA Refseq: NM_000633
• Protein Refseq: NP_000624
• MIM: 151430
• UniProt ID: P10415
• Chromosome Location: 18q21.3
• Pathway: ATF-2 transcriptionfactor network; Activation of BH3-only proteins; Apoptosis; B Cell ReceptorSignaling Pathway; C-MYB transcription factor network; Cholinergic synapse; Colorectalcancer; Direct p53 effectors; EPO signaling pathway; Focal Adhesion; IL-2Signaling Pathway; Immune System; Prostate cancer; Small cell lung cancer; Toxoplasmosis
• Function: BH3 domain binding; channelactivity; identical protein binding; protease binding; protein binding; proteinheterodimerization activity; protein homodimerization activity; proteinphosphatase 2A binding; sequence-specific DNA binding; transcription factorbinding; ubiquitin protein ligase binding