Recombinant Human METTL1, His-tagged, 33.6kDa (296aa)

• Cat.No.: METTL1-953H
• Product Overview: Recombinant human METTL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: METTL1 is known as tRNA (guanine-N (7)-)-methyltransferase which belongs to the methyltransferase superfamily. METTL1 shows high sequence similarity to yeast ORF YDL201w and has been shown to be inactivated by phosphorylation. This protein contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphylation. It catalyzes the formation of N (7)-methylguanine at position 46 (m7G46) in tRNA.
• Form: Liquid. In Phosphate Buffered Saline pH7.4 containing 10% glycerol.
• Molecular Weight: 33.6kDa (296aa) confirmed by MALDI-TOF.
• Purity: > 90% by SDS - PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAAETRNVAG AEAPPPQKRY YRQRAHSNPM ADHTLRYPVK PEEMDWSELY PEFFAPLTQN QSHDDPKDKK EKRAQAQVEF ADIGCGYGGL LVELSPLFPD TLILGLEIRV KVSDYVQDRI RALRAAPAGG FQNIACLRSN AMKHLPNFFY KGQLTKMFFL FPDPHFKRTK HKWRIISPTL LAEYAYVLRV GGLVYTITDV LELHDWMCTH FEEHPLFERV PLEDLSEDPV VGHLGTSTEE GKKVLRNGGK NFPAIFRRIQ DPVLQAVTSQ TSLPGH.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: METTL1 methyltransferase like 1 [ Homo sapiens ]
• Synonyms: methyltransferase like 1; TRM8; C12orf1; YDL201w; FLJ95748; METT- L1;tRNA (guanine-N(7)-)-methyltransfe rase; D1075-like gene product; methy- ltransferase-like 1; tRNA(m7G4- 6)-met hyltransferase; methyltransfera- se-like protein 1; Methyltransferase-like protein 1
• Gene ID: 4234
• mRNA Refseq: NM_005371
• Protein Refseq: NP_005362
• MIM: 604466
• UniProt ID: Q9UBP6
• Chromosome Location: 12p13
• Function: methyltransferase activity; protein binding; tRNA (guanine-N7-)-methyltransferase activity; contributes_to tRNA (guanine-N7-)-methyltransferase activity; tRNA binding; transferase activity