Recombinant Human Methionyl Aminopeptidase 1, His-tagged

• Cat.No.: METAP1-1960H
• Product Overview: Recombinant humanMETAP1 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: METAP1(Methionine aminopeptidase 1) belongs to the peptidase M24A family. Thisprotein removes the amino-terminal methionine from nascent proteins. Itrequired for normal progression through the cell cycle. The active site ofMetAP-1 contains two adjacent divalent metal ions connected by a watermolecule or hydroxide ion.
• Form: Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 3mM DTT, 40% glycerol, 200mM NaCl, 0.1mMPMSF.
• Molecular Weight: 45.7kDa(409aa), confirmed by MALDI-TOF.
• Purity: > 90%by SDS-PAGE
• Concentration: 1 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSMAAVETR VCETDGCSSE AKLQCPTCIK LGIQGSYFCS QECFKGSWATHKLLHKKAKD EKAKREVSSW TVEGDINTDP WAGYRYTGKL RPHYPLMPTR PVPSYIQRPD YADHPLGMSESEQALKGTSQ IKLLSSEDIE GMRLVCRLAR EVLDVAAGMI KPGVTTEEID HAVHLACIAR NCYPSPLNYYNFPKSCCTSV NEVICHGIPD RRPLQEGDIV NVDITLYRNG YHGDLNETFF VGEVDDGARK LVQTTYECLMQAIDAVKPGV RYRELGNIIQ KHAQANGFSV VRSYCGHGIH KLFHTAPNVP HYAKNKAVGV MKSGHVFTIEPMICEGGWQD ETWPDGWTAV TRDGKRSAQF EHTLLVTDTG CEILTRRLDS ARPHFMSQF
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: METAP1 methionyl aminopeptidase1 [ Homo sapiens ]
• Official Symbol: METAP1
• Synonyms: METAP1; methionyl aminopeptidase 1; MAP1A;MetAP1A; methionine aminopeptidase 1; MAP 1; metAP 1; peptidase M 1; EC3.4.11.18; KIAA0094; DKFZp781C0419; OTTHUMP00000219920
• Gene ID: 23173
• mRNA Refseq: NM_015143
• Protein Refseq: NP_055958
• MIM: 610151
• UniProt ID: P53582
• Chromosome Location: 4q23
• Function: aminopeptidase activity; metal ion binding;metalloexopeptidase activity; peptidase activity