Recombinant Human MEF2A 293 Cell Lysate

• Cat.No.: MEF2A-4376HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for myocyte enhancer factor 2A (MEF2A), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: MEF2A myocyte enhancer factor 2A [ Homo sapiens ]
• Official Symbol: MEF2A
• Synonyms: MEF2A; myocyte enhancer factor 2A; myocyte-specific enhancer factor 2A; serum response factor-like protein 1; MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A); mef2; ADCAD1; RSRFC4; RSRFC9
• Gene ID: 4205
• mRNA Refseq: NM_001130926
• Protein Refseq: NP_001124398
• Chromosome Location: 15q26
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Adipogenesis, organism-specific biosystem; CDO in myogenesis, organism-specific biosystem; Developmental Biology, organism-specific biosystem; ERK/MAPK targets, organism-specific biosystem; Energy Metabolism, organism-specific biosystem; Immune System, organism-specific biosystem
• Function: RNA polymerase II regulatory region sequence-specific DNA binding; RNA polymerase II transcription coactivator activity; RNA polymerase II transcription factor binding; SMAD binding; activating transcription factor binding; chromatin binding; histone acetyltransferase binding; histone deacetylase binding; protein binding; contributes_to protein binding; protein heterodimerization activity; protein kinase binding; sequence-specific DNA binding; sequence-specific DNA binding RNA polymerase II transcription factor activity; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity