Recombinant Human MDK Protein (Val21-Asp143), C-His tagged, Animal-free, Carrier-free

• Cat.No.: MDK-216H
• Product Overview: Recombinant Human MDK Protein (Val21-Asp143) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Midkine, also known as neurite growth-promoting factor 2 (NEGF2) is a member of a small family of secreted Growth Factorss, furthermore high expression in lymph node, endometrium, spleen, and colon. Midkine is a 13.5 kDa protein containing 143 amino acids, which promotes angiogenesis, cell growth, migration, and gene expression of different cell types probably via a multiprotein receptor complex consisting of several molecules.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 14.36 kDa.
  The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Val21-Asp143
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 3.5.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: MDK midkine (neurite growth-promoting factor 2) [ Homo sapiens (human) ]
• Official Symbol: MDK
• Synonyms: MDK; midkine (neurite growth-promoting factor 2); NEGF2; midkine; FLJ27379; MK; ARAP; amphiregulin-associated protein; midgestation and kidney protein; neurite outgrowth-promoting protein; neurite outgrowth-promoting factor 2
• Gene ID: 4192
• mRNA Refseq: NM_001012333
• Protein Refseq: NP_001012333
• MIM: 162096
• UniProt ID: P21741