Recombinant Human MDH1 protein, His & GST-tagged

• Cat.No.: MDH1-1546H
• Product Overview: Recombinant Human MDH1 aa. (Ser2~Ala334 (Accession # P40925)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Ser2~Ala334
• Description: This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 66.3kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 6.9

Gene Information

• Gene Name: MDH1 malate dehydrogenase 1 [ Homo sapiens (human) ]
• Official Symbol: MDH1
• Synonyms: MDH1; malate dehydrogenase 1; MDHA; MOR2; MDH-s; HEL-S-32; MGC:1375; malate dehydrogenase, cytoplasmic; malate dehydrogenase, peroxisomal; cytosolic malate dehydrogenase; diiodophenylpyruvate reductase; epididymis secretory protein Li 32; malate dehydrogenase 1, NAD (soluble); soluble malate dehydrogenase
• Gene ID: 4190
• mRNA Refseq: NM_001199111.1
• Protein Refseq: NP_001186040.1
• UniProt ID: P40925