Recombinant Human MC1R 293 Cell Lysate

Description :	Antigen standard for melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor) (MC1R) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source :	HEK 293 cells
Species :	Human
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag :	Non

Gene Name	MC1R melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor) [ Homo sapiens ]
Official Symbol	MC1R
Synonyms	MC1R; melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor); melanocyte-stimulating hormone receptor; MSH R; MC1-R; melanotropin receptor; CMM5; MSH-R; SHEP2; MGC14337;
Gene ID	4157
mRNA Refseq	NM_002386
Protein Refseq	NP_002377
MIM	155555
UniProt ID	Q01726
Chromosome Location	16q24.3
Pathway	Chaperonin-mediated protein folding, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Cooperation of Prefoldin and TriC/CCTin actin and tubulin folding, organism-specific biosystem; Diurnally regulated genes with circadian orthologs, organism-specific biosystem; Formation of tubulin folding intermediates by CCT/TriC, organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem;
Function	G-protein coupled peptide receptor activity; melanocortin receptor activity; melanocyte-stimulating hormone receptor activity; protein binding; receptor activity; signal transducer activity; ubiquitin protein ligase binding;

COA