Recombinant Human Maternal Embryonic Leucine Zipper Kinase, GST-tagged

• Cat.No.: MELK-1059H
• Product Overview: Recombinant human MELK (1-340) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-340 a.a.
• Description: MELK or maternal embryonic leucine zipper kinase is a member of the CAMKL kinase family. MELK is a key regulator of the proliferation of malignant brain tumors including their stem cells (1). MELK transcript abundance correlates with malignancy grade in human astrocytomas and represents a therapeutic target for the management of the most frequent brain tumors in adult and children. MELK also plays a role in mammary carcinogenesis through inhibition of the pro-apoptotic function of Bcl-GL (2). Therefore, the kinase activity of MELK could be a promising molecular target for development of therapy for patients with breast cancers.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 61 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol
• Specific Activity: 200 nmol/min/mg
• Purity: > 85%
• Concentration: 0.1 ug/ul
• Sequences: 1-340
• Storage: Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: MELK maternal embryonic leucine zipper kinase [ Homo sapiens ]
• Official Symbol: MELK
• Synonyms: MELK; maternal embryonic leucine zipper kinase; HPK38; KIAA0175; Hmelk; pEg3 kinase; protein kinase PK38; EC 2.7.11.1; Protein kinase PK38
• Gene ID: 9833
• mRNA Refseq: NM_014791
• Protein Refseq: NP_055606
• MIM: 607025
• UniProt ID: Q14680
• Chromosome Location: 9p13.2
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity