Recombinant Human MAPKAPK5 Protein (S2-Q473), Tag Free

• Cat.No.: MAPKAPK5-1303H
• Product Overview: Recombinant Human GG-MAPKAPK5(S2-Q473 end) Protein was expressed in Insect cell.

Specification

• Description: Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.
• Source: Insect cell
• Species: Human
• Tag: Non
• Form: Liquid
• Protein length: S2-Q473
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50mM Tris-HCl (pH 7.5), 200mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: MAPKAPK5 mitogen-activated protein kinase-activated protein kinase 5 [ Homo sapiens (human) ]
• Official Symbol: MAPKAPK5
• Synonyms: MAPKAPK5; mitogen-activated protein kinase-activated protein kinase 5; MAP kinase-activated protein kinase 5; PRAK; MAPKAPK-5; MAPKAP kinase 5; MAPK-activated protein kinase 5; p38-regulated/activated protein kinase; MK5; MK-5; MAPKAP-K5
• Gene ID: 8550
• mRNA Refseq: NM_003668
• Protein Refseq: NP_003659
• MIM: 606723
• UniProt ID: Q8IW41