Recombinant Human MAPKAPK5

• Cat.No.: MAPKAPK5-736H
• Product Overview: Recombinant Human MAPKAPK5, full length, amino acids M1-Q471, activated, untagged, was expressed inE.coli. MW = 54,161 Da.

Specification

• Cat. No.: MAPKAPK5-736H
• Description: MAP kinase-activated protein kinase 5 is an enzyme that in humans is encoded by the MAPKAPK5 gene.The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites.
• Source: E.coli.
• Purification: Immobilized Metal Affinity Chromatography.
• Product Identity: MAPKAPK5 was confirmed as MAPKAPK5 by mass spectroscopy LC-ESI-MS/MS.
• Activation: with MKK6-SDTD / p38-alpha.
• Storage Buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 20% glycerol.
• Concentration: 0.772 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
• Specific Activity: 11 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!
• Tag: Non
• Protein length: 1-471 a.a.
• Full Length: Full L.

Gene Information

• Gene Name: MAPKAPK5 mitogen-activated protein kinase-activated protein kinase 5 [ Homo sapiens ]
• Synonyms: MAPKAPK5; mitogen-activated protein kinase-activated protein kinase 5; PRAK; MAP kinase-activated protein kinase 5; MAPKAPK-5; MAPKAP kinase 5; MAPK-activated protein kinase 5; p38-regulated/activated protein kinase; EC 2.7.11.1
• Gene ID: 8550
• mRNA Refseq: NM_003668
• Protein Refseq: NP_003659
• MIM: 606723
• UniProt ID: Q8IW41
• Chromosome Location: 12q24.12-q24.13
• Pathway: MAPK signaling pathway
• Function: ATP binding; MAP kinase kinase activity; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity