Recombinant Human MAP3K8, GST-His

• Cat.No.: MAP3K8-636H
• Product Overview: Recombinant human Cot/Tpl2 (a.a. 30-397) with N-terminal GST-His6-tag was expressed in aBaculovirus infected Sf9 cellexpression system. MW = 70 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 30-397 a.a.
• Description: Mitogen-activated protein kinase kinase kinase 8 is an enzyme that in humans is encoded by the MAP3K8 gene. This gene was identified by its oncogenic transforming activity in cells. The encoded protein is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB.
• Purity: >80%.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 3 mM DTT.
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Stability: >6 months at –80°C.
• Pathways: MAPK signaling pathway; T cell receptor signaling pathway; Toll-like receptor signaling pathway; Signaling in Immune system

Gene Information

• Gene Name: MAP3K8 mitogen-activated protein kinase kinase kinase 8 [ Homo sapiens ]
• Synonyms: mitogen-activated protein kinase kinase kinase 8; COT; EST; ESTF; TPL2; MEKK8; Tpl-2; c-COT; FLJ10486; MAP3K8; proto-oncogene c-Cot; tumor progression locus 2; tumor progression locus-2; Ewing sarcoma transformant; Cancer Osaka thyroid oncogene; cot (cancer Osaka thyroid) oncogene; serine/threonine-protein kinase cot; proto-oncogene serine/threoine protein kinase; OTTHUMP00000019393; Ewing sarcoma transformant; OTTHUMP00000019392; TPL-2; Tumor progression locus 2; COT proto-oncogene serine/threonine-protein kinase; EC 2.7.11.25; C-COT
• Gene ID: 1326
• mRNA Refseq: NM_005204
• Protein Refseq: NP_005195
• MIM: 191195
• UniProt ID: P41279
• Chromosome Location: 10p11.23
• Function: protein serine/threonine kinase activity; MAP kinase kinase kinase activity; ATP binding; magnesium ion binding; nucleotide binding; protein binding; transferase activity