Active Recombinant Human MAP3K8, GST-tagged

• Cat.No.: MAP3K8-572H
• Product Overview: Recombinant Human MAP3K8 (Accession # NM_005204) amino acids 30 - 397, fused with an N-terminal GST tag, was produced in baculovirus in Sf 9 insect cells.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 30-397 a.a.
• Form: Supplied in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
• Bio-activity: The specific activity of COT was determined to be 1150 nmol/min/mg using MEK1 and ERK1 substrates and a myelin basic protein (MBP) substrate
• Molecular Mass: In SDS-PAGE migrates as 70 kDa.
• Purity: The purity was determined to be >90% by densitometry
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: MAP3K8 mitogen-activated protein kinase kinase kinase 8 [ Homo sapiens ]
• Official Symbol: MAP3K8
• Synonyms: MAP3K8; mitogen-activated protein kinase kinase kinase 8; COT, ESTF; c COT; EST; MEKK8; Tpl 2; proto-oncogene c-Cot; tumor progression locus 2; Ewing sarcoma transformant; cot (cancer Osaka thyroid) oncogene; proto-oncogene serine/threoine protein kinase; COT; ESTF; TPL2; Tpl-2; c-COT; FLJ10486
• Gene ID: 1326
• mRNA Refseq: NM_001244134
• Protein Refseq: NP_001231063
• MIM: 191195
• UniProt ID: P41279
• Chromosome Location: 10p11.2