Recombinant Human MAP/Microtubule Affinity-Regulating Kinase 3, GST-His-tagged

• Cat.No.: MARK3-5450H
• Product Overview: Recombinant humanfull length MARK3 (amino acids M1-L713) N-terminally fused to GST-His6-Thrombincleavage site.

Specification

• Cat. No.: MARK3-5450H
• Description: MARK3 encoded bythis gene is activated by phosphorylation and in turn is involved in thephosphorylation of tau proteins MAP2 and MAP4. Several transcript variantsencoding different isoforms have been found for this gene.
• Source: Sf9 insect cells
• Species: Human
• Form: Liquid in 50 mMHEPES, pH 7.5 + 100 mM NaCl + 5 mM DTT + 4 mM reduced glutathione + 20%glycerol
• Molecular Weight: 109.4 kDa
• Concentration: 0.082 mg/ml
• Purity: Affinity purifiedusing GSH-agarose
• Specific Activity: 18 pmol/µg x min. Assaybuffer: 60 mM HEPES-NaOH, pH 7.5 + 3 mM MgCl2 + 3 mM MnCl2 + 3 µMNa-orthovanadate + 1.2 mM DTT + 2.5 µg/50 µl PEG20.000 + ATP (variable) +0.25 µg/50 µl CHKtide substrate + 200 ng/50 µl MARK3.
• Storage: Store in workingaliquots at -80°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: MARK3
• Tag: His&GST
• Protein length: 1-713 a.a.
• Full Length: Full L.

Gene Information

• Gene Name: MARK3 MAP/microtubuleaffinity-regulating kinase 3 [ Homo sapiens ]
• Synonyms: MARK3;MAP/microtubule affinity-regulating kinase 3; KP78; CTAK1; PAR1A; Par-1a;EMK-2; C-TAK1; OTTHUMP00000246863; OTTHUMP00000246864; OTTHUMP00000246865;OTTHUMP00000246866; OTTHUMP00000246867; OTTHUMP00000246868; ELKL motif kinase2; protein kinase STK10; ser/Thr protein kinase PAR-1; cdc25C-associatedprotein kinase 1; rine/threonine-protein kinase p78; EC 2.7.11.1
• Gene ID: 4140
• mRNA Refseq: NM_002376
• Protein Refseq: NP_002367
• MIM: 602678
• UniProt ID: P27448
• Chromosome Location: 14q32.3
• Function: ATP binding;nucleotide binding; protein binding; protein serine/threonine kinaseactivity; transferase activity, transferring phosphorus-containing groups