Recombinant Human MAOA Protein, 2-497, N-His and N-FLAG tagged

• Cat.No.: MAOA-15H
• Product Overview: Recombinant human MAOA (monoamine oxidase A), encompassing amino acids 2-497. This construct contains an N-terminal His-tag (6xHis) followed by a Flag-tag was expressed in Sf9. The recombinant protein was affinity purified.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: N-His and N-FLAG
• ProteinLength: 2-497
• Description: Monoamine Oxidases (MAO) catalyze the oxidation of monoamines such as dopamine, serotonin and adrenalin. They are found bound to the outer membrane of mitochondria in most cell types in the body. Two subtypes of monoamine oxidase have been identified: MAO-A and MAO-B. Both are found in neurons and astroglia, with MAO-A also found in the liver, gastrointestinal tract and placenta and MAO-B in blood platelets. Abnormal regulation of MAOs in the body has been associated with depression, substance abuse, attention deficit disorder, and irregular sexual maturation. Mutation of this gene results in Brunner syndrome. This gene has also been associated with a variety of other psychiatric disorders, including antisocial behavior. Alternatively spliced transcript variants encoding multiple isoforms have been observed.
• Form: Aqueous buffer solution
• Molecular Mass: 58 kDa
• Purity: ≥90%
• Notes: Avoid freeze/thaw cycles
• Usage: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
• Storage: At least 6 months at –80 centigrade.
• Storage Buffer: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 100 μg/mL FLAG Peptide
• Shipping: At -80 centigrade
• References: 1. Hodorova, I., et al., Med. Sci. Monit. 2012;18(12): BR482-BR486.
  2. Brunner, H.G., et al., Science. 1993;262(5133): 578-580.

Gene Information

• Gene Name: MAOA monoamine oxidase A [ Homo sapiens (human) ]
• Official Symbol: MAOA
• Synonyms: MAOA; monoamine oxidase A; amine oxidase [flavin-containing] A; MAO-A; monoamine oxidase type A
• Gene ID: 4128
• mRNA Refseq: NM_000240
• Protein Refseq: NP_000231
• MIM: 309850
• UniProt ID: P21397