Recombinant Human MAN1A1 protein, His & GST-tagged

• Cat.No.: MAN1A1-1549H
• Product Overview: Recombinant Human MAN1A1 aa. (Ile322~Glu653 (Accession # P33908)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Ile322~Glu653
• Description: This gene encodes a class I mammalian Golgi 1,2-mannosidase which is a type II transmembrane protein. This protein catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides and belongs to family 47 of glycosyl hydrolases.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 68.1kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 5.8

Gene Information

• Gene Name: MAN1A1 mannosidase alpha class 1A member 1 [ Homo sapiens (human) ]
• Official Symbol: MAN1A1
• Synonyms: MAN1A1; mannosidase alpha class 1A member 1; MAN9; HUMM3; HUMM9; mannosyl-oligosaccharide 1,2-alpha-mannosidase IA; Man9-mannosidase; alpha-1,2-mannosidase IA; man(9)-alpha-mannosidase; processing alpha-1,2-mannosidase IA
• Gene ID: 4121
• mRNA Refseq: NM_005907.3
• Protein Refseq: NP_005898.2
• UniProt ID: P33908