Recombinant human MAGED1, His-tagged

• Cat.No.: MAGED1-2455H
• Product Overview: Recombinant human MAGED1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: MAGED1, as known as Melanoma-associated antigen D1, is expressed in almost all normal adult tissues. It has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. It has been involved in Prader-Willi syndrome, which includes hyperphagia, repetitive and compulsive behaviors, and cognitive impairment.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 1mM DTT
• Molecular Mass: 31.7kDa (280aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSLRPSPNS RASQNPGAAQ PRDVALLQER ANKLVKYLML KDYTKVPIKR SEMLRDIIRE YTDVYPEIIERACFVLEKKF GIQLKEIDKE EHLYILISTP ESLAGILGTT KDTPKLGLLL VILGVIFMNG NRASEAVLWE ALRKMGLRPG VRHPLLGDLRKLLTYEFVKQ KYLDYRRVPN SNPPEYEFLW GLRSYHETSK MKVLRFIAEV QKRDPRDWTA QFMEAADEAL DALDAAAAEA EARAEARTRMGIGDEAVSGP
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: MAGED1 melanoma antigen family D, 1 [ Homo sapiens ]
• Official Symbol: MAGED1
• Synonyms: MAGED1; melanoma antigen family D, 1; melanoma-associated antigen D1; DLXIN 1; NRAGE; MAGE-D1 antigen; MAGE tumor antigen CCF; neurotrophin receptor-interacting MAGE homolog; DLXIN-1
• Gene ID: 9500
• mRNA Refseq: NM_001005332
• Protein Refseq: NP_001005332
• MIM: 300224
• UniProt ID: Q9Y5V3
• Chromosome Location: Xp11.23
• Pathway: Apoptosis, organism-specific biosystem; Cell death signalling via NRAGE, NRIF and NADE, organism-specific biosystem; NRAGE signals death through JNK, organism-specific biosystem; Neurotrophic factor-mediated Trk receptor signaling, organism-specific biosystem; Neurotrophin signaling pathway, organism-specific biosystem; Neurotrophin signaling pathway, conserved biosystem; Regulation of Apoptosis, organism-specific biosystem
• Function: protein binding