Recombinant Human MAGED1 293 Cell Lysate

• Cat.No.: MAGED1-4540HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for melanoma antigen family D, 1 (MAGED1), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: MAGED1 melanoma antigen family D, 1 [ Homo sapiens ]
• Official Symbol: MAGED1
• Synonyms: MAGED1; melanoma antigen family D, 1; melanoma-associated antigen D1; DLXIN 1; NRAGE; MAGE-D1 antigen; MAGE tumor antigen CCF; neurotrophin receptor-interacting MAGE homolog; DLXIN-1
• Gene ID: 9500
• mRNA Refseq: NM_001005332
• Protein Refseq: NP_001005332
• MIM: 300224
• UniProt ID: Q9Y5V3
• Chromosome Location: Xp11.23
• Pathway: Apoptosis, organism-specific biosystem; Cell death signalling via NRAGE, NRIF and NADE, organism-specific biosystem; NRAGE signals death through JNK, organism-specific biosystem; Neurotrophic factor-mediated Trk receptor signaling, organism-specific biosystem; Neurotrophin signaling pathway, organism-specific biosystem; Neurotrophin signaling pathway, conserved biosystem; Regulation of Apoptosis, organism-specific biosystem
• Function: protein binding