Recombinant Human MADD Protein, His-tagged

• Cat.No.: MADD-2409H
• Product Overview: Recombinant Human MADD Protein (Asn1388-Ser1647) with a N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Asn1388-Ser1647
• Description: Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. The protein encoded by this gene is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.6 kDa
  Accurate Molecular Mass: 34 kDa
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: MADD MAP-kinase activating death domain [ Homo sapiens (human) ]
• Official Symbol: MADD
• Synonyms: MADD; MAP-kinase activating death domain; MAP kinase-activating death domain protein; DENN; KIAA0358; RAB3GEP; Rab3 GDP/GTP exchange factor; insulinoma glucagonoma clone 20; differentially expressed in normal and neoplastic cells; IG20; FLJ35600; FLJ36300
• Gene ID: 8567
• mRNA Refseq: NM_001135943
• Protein Refseq: NP_001129415
• MIM: 603584
• UniProt ID: Q8WXG6