Recombinant Human MADCAM1 protein, His-tagged

• Cat.No.: MADCAM1-3980H
• Product Overview: Recombinant Human MADCAM1 is produced by our Mammalian expression system and the target gene encoding Gln19-Gln333 is expressed with a 6His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Gln19-Gln333
• Description: Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is an approximately 60 kDa type 1 transmembrane glycoprotein. It is an endothelial cell adhesion molecule that belongs to the immunoglobulin (Ig) superfamily of proteins. The Ig domains of MAdCAM-1 are critical to alpha (4) beta (7) binding, and the mucin domain has activity in L Selectin binding. MAdCAM-1 expression is up-regulated by TNF-alpha and IL 1 beta. Mucosal addressin cell adhesion molecule (MAdCAM-1) plays a pivotal role in T-lymphocyte homing to the gut. MAdCAM 1 expression is also dramatically increased in chronic inflammatory and disease states, including inflammatory bowel disease (Crohn’s disease and ulcerative colitis), sclerosing cholangitis, and diabetes, and may play an important role in these conditions.
• Form: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Molecular Mass: 55-65kDa, reducing conditions.
• Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
  Aliquots of reconstituted samples are stable at < -20°C for 3 months.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
  It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Gene Information

• Gene Name: MADCAM1 mucosal vascular addressin cell adhesion molecule 1 [ Homo sapiens ]
• Official Symbol: MADCAM1
• Synonyms: MADCAM1; mucosal vascular addressin cell adhesion molecule 1; MACAM1; MAdCAM-1; hMAdCAM-1
• Gene ID: 8174
• mRNA Refseq: NM_130760.2
• Protein Refseq: NP_570116.2
• MIM: 102670
• UniProt ID: Q13477