Active Recombinant Human Mucosal Vascular Addressin Cell Adhesion Molecule 1, Fc-tagged

• Cat.No.: MADCAM1-5684H
• Product Overview: Recombinant Human MADCAM1protein was expressed in Murine myeloma cell line with the Fc part of humanIgG1 Pro100-Lys330 (N-terminus) and Met1-Gln333 (C-terminus).

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc
• Protein Length: 1-333 a.a.
• Description: MADCAM1 is anapproximately 60 kDa type 1 transmembrane glycoprotein. It is an endothelialcell adhesion molecule that belongs to the immunoglobulin) superfamily ofproteins. Human MADCAM1 is synthesized as a 382 amino acid precursor thatcontains an 18 aa signal sequence, a 299 aa extracellular domain, a 21 aatransmembrane segment, and a 44 aa cytoplasmic tail. Within the ECD there isone potential site for N-linked glycosylation. The ECD comprises two Ig-likedomains of 90 aa and 119 aa, respectively, each possessing invariant cysteineresidues that stabilize the Ig loop.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Val23 & Glu29
• Molecular Weight: 59.3 kDa
• Activity: Measuredby the ability of the immobilized protein to support the adhesion of HUT78human mature cutaneous T cell lymphoma cells. When 5 x 104cells/well are added to recombinant human MADCAM1/Fc Chimera coated plates(2.5 μg/mL with 100 L/well), approximately 50%-70% will adhere after 1 hourincubation at 37°C in the presence of 1 mM MnCl2.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 85-100kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 200μg/mL in PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: MADCAM1 mucosal vascularaddressin cell adhesion molecule 1 [ Homo sapiens ]
• Official Symbol: MADCAM1
• Synonyms: MADCAM1; mucosalvascular addressin cell adhesion molecule 1; MACAM1; mucosal addressin celladhesion molecule 1; MAdCAM-1; hMAdCAM-1; mucosal addressin cell adhesionmolecule-1
• Gene ID: 8174
• mRNA Refseq: NM_130760
• Protein Refseq: NP_570116
• MIM: 102670
• UniProt ID: Q13477
• Chromosome Location: 19p13.3
• Pathway: Adaptive ImmuneSystem; Cell adhesion molecules (CAMs); Immune System; Immunoregulatoryinteractions between a Lymphoid and a non-Lymphoid cell; Integrin cellsurface interactions; Intestinal immune network for IgA production;Paxillin-independent events mediated by a4b1 and a4b7; Signal Transduction