Recombinant Human Lymphotoxin Alpha (TNF superfamily, member 1), HQ-tagged

• Cat.No.: LTA-1253H
• Product Overview: Recombinanthuman LTA contains 171 amino acids and a 16 aa HQ tag. Total length is 187aa.

Specification

• Species: Human
• Source: Hordeum Vulgare
• Tag: HQ
• Description: LTAis a protein that in humans is encoded by the LTA gene. LTA is a member ofthe tumor necrosis factor family, is a cytokine produced by lymphocytes. LTAis highly inducible, secreted, and exists as homotrimeric molecule. LTA formsheterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to thecell surface. LTA mediates a large variety of inflammatory,immunostimulatory, and antiviral responses. LTA is also involved in theformation of secondary lymphoid organs during development and plays a role inapoptosis.
• Form: Lyophilized from a0.2 μm sterile filtered solution of PBS, pH 7.2.
• Endotoxin Level: <0.005 ng per μg of product (0.05 EU/μg) as measured by turbidimetric kineticassay.
• Molecular Weight: PredictedMW = 20.8 kDa. The glycosylated recombinant protein migrates as three bandswith an apparent MW = 20, 23 and 27 kDa in SDS-PAGE.
• Cross-Reactivity: LTA isnot species specific and human LTA is active on mouse cells with a slightly reducedspecific activity.
• Biological Activity: The ED50is determined by the cytotoxicity Assay for the human LTA for dose dependent cytolysiseffects of serial dilutions of LTA on the fibro sarcoma L-929 cells. The ED50lies in the range of 1.0-1.6 ng/ml.
• Reconstitution: Centrifugevial prior to opening. First add sterile distilled water to the vial to fullysolubilize the protein to a concentration not less than 100 μg/ml. Aftercomplete solubilization of the protein, it can be further diluted to otheraqueous solutions.
• Storage: Thelyophilized protein, though stable at room temperature for two weeks, is beststored at -20°C. Reconstituted protein should be used immediately or storedin working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: LTA

Gene Information

• Gene Name: LTA lymphotoxin alpha (TNFsuperfamily, member 1) [ Homo sapiens ]
• Synonyms: LTA; lymphotoxinalpha (TNF superfamily, member 1); TNFB; LT; TNFSF1; OTTHUMP00000029184; OTTHUMP00000165897;LT-alpha; TNF-beta; Tumor necrosis factor ligandsuperfamily member 1; lymphotoxin alpha; Tumornecrosis factor beta
• Gene ID: 4049
• mRNA Refseq: NM_000595
• Protein Refseq: NP_000586
• MIM: 153440
• UniProt ID: P01374
• Chromosome Location: 6p21.3
• Pathway: Apoptosis; Cytokine-cytokinereceptor interaction; HTLV-I infection; Herpes simplex infection; IL2signaling events mediated by STAT5; L4-mediated signaling events; Type Idiabetes mellitus
• Function: cytokine activity; receptorbinding; tumor necrosis factor receptor binding