Recombinant Human LSM4, His-tagged

• Cat.No.: LSM4-3398H
• Product Overview: Recombinant human LSM4 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW = 17.5 kDa (159aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: LSM4, also called U6 snRNA-associated Sm-like protein or glycine-rich protein (GRP), forms donut-shaped heptameric complexes that are involved in various steps of RNA metabolism. It facilitates RNA protein interactions and structural changes that are required during ribosomal subunit assembly. It binds specifically to the 3"-terminal U-tract of U6 snRNA.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl, 1mM DTT.
• Purity: > 90% by SDS - PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MLPLSLLKTA QNHPMLVELK NGETYNGHLV SCDNWMNINL REVICTSRDG DKFWRMPECY IRGSTIKYLR IPDEIIDMVK EEVVAKGRGR GGLQQQKQQK GRGMGGAGRG VFGGRGRGGI PGTGRGQPEK KPGRQAGKQ.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: RNA degradation; Spliceosome; Metabolism of RNA

Gene Information

• Gene Name: LSM4 LSM4 homolog, U6 small nuclear RNA associated (S. cerevisiae) [ Homo sapiens ]
• Synonyms: LSM4 homolog, U6 small nuclear RNA associated (S. cerevisiae); YER112W; LSM4; U6 snRNA-associated Sm-like protein LSm4; GRP; glycine-rich protein;Glycine-rich protein
• Gene ID: 25804
• mRNA Refseq: NM_012321
• Protein Refseq: NP_036453
• MIM: 607284
• UniProt ID: Q9Y4Z0
• Chromosome Location: 19p13.11
• Function: RNA binding; protein binding