Recombinant Human LSM1, His-tagged

• Cat.No.: LSM1-2818H
• Product Overview: Recombinant human LSM1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 17.3kDa (153aa), confirmed by MALDI-TOF.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: U6 snRNA-associated Sm-like protein LSm1, also known as LSM1 plays a role in replication-dependent histone mRNA degradation and binds specifically to the 3"-terminal U-tract of U6 snRNA. LSM1 protein facilitates RNA protein interactions and structural changes that are required during ribosomal subunit assembly. LSM1 is naturally overexpressed in pancreatic cancer as well as in certain breast cancer cell lines.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MNYMPGTASL IEDIDKKHLV LLRDGRTLIG FLRSIDQFAN LVLHQTVERI HVGKKYGDIP RGIFVVRGEN VVLLGEIDLE KESDTPLQQV SIEEILEEQR VEQQTKLEAE KLKVQALKDR GLSIPRADTL DEY
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol, 0.1M NaCl.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: LSM1 LSM1 homolog, U6 small nuclear RNA associated (S. cerevisiae) [ Homo sapiens ]
• Synonyms: LSM1; LSM1 homolog, U6 small nuclear RNA associated (S. cerevisiae); CASM; YJL124C; U6 snRNA-associated Sm-like protein LSm1; cancer-associated Sm-like; small nuclear ribonuclear CaSm
• Gene ID: 27257
• mRNA Refseq: NM_014462
• Protein Refseq: NP_055277
• MIM: 607281
• UniProt ID: O15116
• Chromosome Location: 8p11.2
• Pathway: RNA degradation
• Function: RNA binding; protein binding