Recombinant Human LPAR3 293 Cell Lysate

• Cat.No.: LPAR3-4673HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for lysophosphatidic acid receptor 3 (LPAR3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: LPAR3 lysophosphatidic acid receptor 3 [ Homo sapiens ]
• Official Symbol: LPAR3
• Synonyms: LPAR3; lysophosphatidic acid receptor 3; EDG7, endothelial differentiation, lysophosphatidic acid G protein coupled receptor, 7; Edg 7; HOFNH30; LP A3; LPA3; RP4 678I3; LPA-3; LPA receptor 3; LPA receptor EDG7; lysophosphatidic acid receptor Edg-7; endothelial cell differentiation gene 7; calcium-mobilizing lysophosphatidic acid receptor LP-A3; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 7; EDG7; GPCR; Edg-7; LP-A3; RP4-678I3; FLJ98231
• Gene ID: 23566
• mRNA Refseq: NM_012152
• Protein Refseq: NP_036284
• MIM: 605106
• UniProt ID: Q9UBY5
• Chromosome Location: 1p22.3
• Pathway: Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; LPA receptor mediated events, organism-specific biosystem; Lysosphingolipid and LPA receptors, organism-specific biosystem
• Function: G-protein alpha-subunit binding; G-protein coupled receptor activity; lipid binding; lysophosphatidic acid receptor activity; phospholipid binding; receptor activity; signal transducer activity