Active Recombinant Human LBP, His-tagged

• Cat.No.: LBP-152H
• Product Overview: The Lipopolysaccharide Binding Protein is produced from human LBP transfected CHO-cells in serum free medium. Before transfection the complete human LBP-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. The recombinant Human LBP was purified by his-tag with metal affinity purification with Talon and controlled by SDS page.Attention: His-tag has no protease site and can not be split off.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Description: The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP).
• Form: Sterile Filtered White lyophilized (freeze-dried) powder. Recombinant Human LBP was lyophilized from a protein solution (0.25 mg/ml) containing phosphate-buffered saline, pH 7.2.
• Bio-activity: Up to 0.2 µg/ ml LBP mediates binding of FITC-LPS (0.5µg/ml) to CD14+CHO transfectants (FACS).
• Molecular Mass: On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa.
• Stability: Lyophilized LBP Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
• Reconstitution: Every 10µg of recombinant human LBP should be reconstituted using 40µl of sterile H2O. The solution can be than diluted with phosphate-buffered saline or other buffers.

Gene Information

• Gene Name: LBP lipopolysaccharide binding protein [ Homo sapiens (human) ]
• Official Symbol: LBP
• Synonyms: LBP; lipopolysaccharide binding protein; MGC22233; lipopolysaccharide-binding protein; LPS-binding protein; OTTHUMP00000030965
• Gene ID: 3929
• mRNA Refseq: NM_004139
• Protein Refseq: NP_004130
• MIM: 151990
• UniProt ID: P18428
• Chromosome Location: 20q11.23
• Pathway: IL6-mediated signaling events; NF-kappa B signaling pathway; Toll Like Receptor 4 (TLR4) Cascade
• Function: lipopolysaccharide binding; lipoteichoic acid binding; protein binding; receptor binding