Recombinant Human LIPG Protein, His-tagged

• Cat.No.: LIPG-045H
• Product Overview: Recombinant human LIPG / EDL (rhLIPG) Ser21-Pro500 (Accession # AAH60825.1 ) fused with a N-terminal his tag was produced in human HEK293 cells.

Specification

• Description: The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq, Jul 2008]
• Source: HEK293 cells
• Species: Human
• Tag: His
• Molecular Mass: rhLIPG, fused with 6×his tag at the N-terminal and has a calculated MW of 55.4 kDa expressed. Protein migrates as 55-65 kDa in reduced SDS-PAGE resulting from glycosylation.
• Endotoxin: Less than 1.0 EU per 1 µg of the rhLIPG by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Notes: Research Use Only. Not for use in diagnostic or therapeutic procedures.
• Storage: No activity loss was observed after storage at 4-8 centigrade for 1 month or -20 centigrade for 1 year in lyophilized state. rhLIPG should be aliquoted and stored at -8 centigrade centigrade in liquid formulation, for long term storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as a 0.22 µm filtered solution in PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally mannitol or trehalose are added as protectants before lyophilization. Reconstitution instruction or specific concentrations are included in the hardcopy of CoA along with the products.
• Protein length: 21-500 a.a.

Gene Information

• Gene Name: LIPG lipase, endothelial [ Homo sapiens ]
• Official Symbol: LIPG
• Synonyms: LIPG; lipase, endothelial; endothelial lipase; EDL; lipoprotein lipase H; endothelial cell-derived lipase; EL; PRO719
• Gene ID: 9388
• mRNA Refseq: NM_006033
• Protein Refseq: NP_006024
• MIM: 603684
• UniProt ID: Q9Y5X9