Recombinant Human LIMS1 protein, His & T7-tagged

• Cat.No.: LIMS1-1507H
• Product Overview: Recombinant Human LIMS1 aa. (Ala68~Cys303 (Accession # P48059)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 30.5kDa
• Protein length: Ala68~Cys303
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 8.6

Gene Information

• Gene Name: LIMS1 LIM zinc finger domain containing 1 [ Homo sapiens (human) ]
• Official Symbol: LIMS1
• Synonyms: LIMS1; LIM zinc finger domain containing 1; PINCH; PINCH1; PINCH-1; LIM and senescent cell antigen-like-containing domain protein 1; LIM and senescent cell antigen-like domains 1; LIM-type zinc finger domains 1; particularly interesting new Cys-His protein 1; renal carcinoma antigen NY-REN-48; senescent cell antigen
• Gene ID: 3987
• mRNA Refseq: NM_001193482.1
• Protein Refseq: NP_001180411.1
• UniProt ID: P48059