Recombinant Human Leucine Carboxyl Methyltransferase 1, His-tagged

• Cat.No.: LCMT1-1563H
• Product Overview: Recombinant human LCMT7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.
• Molecular Weight: 41 kDa (358aa), confirmed by MALDI-TOF.
• Purity: > 95% by SDS-PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMATRQR ESSITSCCST SSCDADDEGV RGTCEDASLC KRFAVSIGYW HDPYIQHFVR LSKERKAPEI NRGYFARVHG VSQLIKAFLR KTECHCQIVN LGAGMDTTFW RLKDEDLLPS KYFEVDFPMI VTRKLHSIKC KPPLSSPILE LHSEDTLQMD GHILDSKRYA VIGADLRDLS ELEEKLKKCN MNTQLPTLLI AECVLVYMTP EQSANLLKWA ANSFERAMFI NYEQVNMGDR FGQIMIENLR RRQCDLAGVE TCKSLESQKE RLLSNGWETA SAVDMMELYN RLPRAEVSRI ESLEFLDEME LLEQLMRHYC LCWATKGGNE LGLKEITY
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Histidine metabolism; Tyrosine metabolism

Gene Information

• Gene Name: LCMT1 leucine carboxyl methyltransferase 1 [ Homo sapiens ]
• Official Symbol: LCMT1
• Synonyms: LCMT1; leucine carboxyl methyltransferase 1; LCMT; PPMT1; CGI-68; protein-leucine O-methyltransferase; protein phosphatase methyltransferase 1; EC 2.1.1
• Gene ID: 51451
• mRNA Refseq: NM_001032391
• Protein Refseq: NP_001027563
• MIM: 610286
• UniProt ID: Q9UIC8
• Chromosome Location: 16p12.1
• Function: S-adenosylmethionine-dependent methyltransferase activity; protein C-terminal carboxyl O-methyltransferase activity; protein binding; transferase activity