Recombinant Human LCMT1 293 Cell Lysate

• Cat.No.: LCMT1-4803HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: LCMT1 leucine carboxyl methyltransferase 1 [ Homo sapiens ]
• Official Symbol: LCMT1
• Synonyms: LCMT1; leucine carboxyl methyltransferase 1; CGI 68; PPMT1; protein phosphatase methyltransferase 1; protein-leucine O-methyltransferase; [Phosphatase 2A protein]-leucine-carboxy methyltransferase 1; LCMT; CGI-68
• Gene ID: 51451
• mRNA Refseq: NM_001032391
• Protein Refseq: NP_001027563
• MIM: 610286
• UniProt ID: Q9UIC8
• Chromosome Location: 16p12.1
• Function: S-adenosylmethionine-dependent methyltransferase activity; protein C-terminal carboxyl O-methyltransferase activity; protein binding; transferase activity