Recombinant Human LEP protein, His & GST-tagged

• Cat.No.: LEP-675H
• Product Overview: Recombinant Human LEP aa. (Val22~Cys167) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Val22~Cys167
• Form: Freeze-dried powder
• Molecular Mass: 46kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.7.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: LEP leptin [ Homo sapiens (human) ]
• Official Symbol: LEP
• Synonyms: OB; OBS; LEPD; leptin (murine obesity homolog); leptin (obesity homolog, mouse); obese protein; obese, mouse, homolog of; obesity factor; leptin
• Gene ID: 3952
• mRNA Refseq: NM_000230.2
• Protein Refseq: NP_000221.1
• UniProt ID: P41159