Recombinant Chicken Leptin

• Cat.No.: LEP-08C
• Product Overview: Recombinant Chicken Leptin Binding Domain produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 208 amino acids and having a molecular mass of 24.5 kDa. The Leptin Binding Domain is purified by proprietary chromatographic techniques.

Specification

• Species: Chicken
• Source: E.coli
• Tag: Non
• Description: Leptin (Greek leptos meaning thin) is a 16 kDa protein hormone that plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. It is one of the most important adipose derived hormones.
• Amino Acid Sequence: The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Ile-Asp-Val-Asn-Ile
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045 mM NaHCO3.
• Solubility: The lyophilized Leptin Binding Domain is very soluble in water and most aqueous buffers below and above the isoelectric point.
• Biological Activity: Biological Activity is evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio.
• Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 2.45 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Leptin Binding Domain as a Reference Standard.
• Storage: Lyophilized Leptin Binding Domain although stable at room temperature, should be stored desiccated below 0℃. Reconstituted Leptin is best stored refrigerated at 4℃.Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: LEP leptin [ Gallus gallus ]
• Synonyms: OB Protein; Obesity Protein; OBS; Obesity factor; LEP; Lep; leptin; ob; obese; obesity factor; leptin (murine obesity homolog); leptin (obesity homolog, mouse); FLJ94114; Obese protein
• Gene ID: 373955