Recombinant Human LEO1 293 Cell Lysate

Description :	Antigen standard for Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source :	HEK 293 cells
Species :	Human
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag :	Non

Gene Name	LEO1 Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol	LEO1
Synonyms	LEO1; Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae); RNA polymerase-associated protein LEO1; replicative senescence down-regulated leo1-like protein; RDL;
Gene ID	123169
mRNA Refseq	NM_138792
Protein Refseq	NP_620147
MIM	610507
UniProt ID	Q8WVC0
Chromosome Location	15q21.2
Function	protein binding;