Recombinant Human Lefty1 Protein, His/GST-tagged

• Cat.No.: PARN-372H
• Product Overview: Recombinant Human Lefty1(Lys178~Asp245) fused with His/GST tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Lys178~Asp245
• Description: The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 38.1kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others

Gene Information

• Gene Name: PARN poly(A)-specific ribonuclease [ Homo sapiens ]
• Official Symbol: PARN
• Synonyms: PARN; poly(A)-specific ribonuclease; poly(A) specific ribonuclease (deadenylation nuclease); poly(A)-specific ribonuclease PARN; DAN; deadenylation nuclease; deadenylating nuclease; polyadenylate-specific ribonuclease; poly(A)-specific ribonuclease (deadenylation nuclease)
• Gene ID: 5073
• mRNA Refseq: NM_001134477
• Protein Refseq: NP_001127949
• MIM: 604212
• UniProt ID: O95453