Recombinant Human LDHB Protein, His-tagged

• Cat.No.: LDHB-931H
• Product Overview: Recombinant Human LDHB(Ala2-Leu334), transcript variant 2, fused with His tag at C-terminal was expressed in E. coli.

Specification

• Description: This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.
• Source: E. coli
• Tag: His
• Form: 20mM Tris-HCl, 100mM NaCl, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
• Molecular Mass: 38.8 kDa
• Endotoxin: < 0.1 EU per µg protein as determined by LAL test
• Purity: >95% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method
• Protein length: 2-334 a.a.

Gene Information

• Gene Name: LDHB lactate dehydrogenase B [ Homo sapiens ]
• Official Symbol: LDHB
• Synonyms: LDHB; lactate dehydrogenase B; L-lactate dehydrogenase B chain; LDH heart subunit; lactate dehydrogenase H chain; renal carcinoma antigen NY-REN-46; LDH-B; LDH-H; LDHBD; TRG-5
• Gene ID: 3945
• mRNA Refseq: NM_001174097
• Protein Refseq: NP_001167568
• MIM: 150100
• UniProt ID: P07195