Recombinant Human KRT81 protein, His-tagged

• Cat.No.: KRT81-1508H
• Product Overview: Recombinant Human KRT81 aa. (Gln142~Thr257 (Accession # Q14533)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB3 and KRTHB6, is found primarily in the hair cortex. Mutations in this gene and KRTHB6 have been observed in patients with a rare dominant hair disease, monilethrix.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 15kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Gln142~Thr257
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 4.9

Gene Information

• Gene Name: KRT81 keratin 81 [ Homo sapiens (human) ]
• Official Symbol: KRT81
• Synonyms: KRT81; keratin 81; HB1; Hb-1; KRTHB1; MLN137; ghHkb1; hHAKB2-1; keratin, type II cuticular Hb1; K81; MLN 137; ghHb1; hair keratin K2.9; hard keratin, type II, 1; keratin 81, type II; keratin, hair, basic, 1; metastatic lymph node 137 gene protein; type II hair keratin Hb1; type-II keratin Kb21
• Gene ID: 3887
• mRNA Refseq: NM_002281.3
• Protein Refseq: NP_002272.2
• UniProt ID: Q14533