Recombinant Human KRT4 protein, His-tagged

• Cat.No.: KRT4-2694H
• Product Overview: Recombinant Human KRT4 aa. (Arg152~Leu457) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 39kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Arg152~Leu457
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 5.7.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: KRT4 keratin 4 [ Homo sapiens (human) ]
• Official Symbol: KRT4
• Synonyms: K4; CK4; CK-4; CYK4; WSN1; cytokeratin 4; keratin 4, type II; type-II keratin Kb4; keratin, type II cytoskeletal 4
• Gene ID: 3851
• mRNA Refseq: NM_002272.3
• Protein Refseq: NP_002263.3
• UniProt ID: B4DRS2