Recombinant Human KREMEN1 protein, His & T7-tagged

• Cat.No.: KREMEN1-7883H
• Product Overview: Recombinant Human KREMEN1 aa. (Lys157~Gly390 (Accession # Q96MU8)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT)/beta-catenin signaling. The encoded protein is a component of a membrane complex that modulates canonical WNT signaling through lipoprotein receptor-related protein 6 (LRP6). It contains extracellular kringle, WSC, and CUB domains. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.3kDa.
• Protein length: Lys157~Gly390
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: KREMEN1 kringle containing transmembrane protein 1 [ Homo sapiens (human) ]
• Official Symbol: KREMEN1
• Synonyms: KREMEN1; kringle containing transmembrane protein 1; KRM1; ECTD13; KREMEN; kremen protein 1; dickkopf receptor; kringle domain-containing transmembrane protein 1; kringle-coding gene marking the eye and the nose; kringle-containing protein marking the eye and the nose
• Gene ID: 83999
• mRNA Refseq: NM_001039570.2
• Protein Refseq: NP_001034659.2
• UniProt ID: Q96MU8