Recombinant Human KRAS(Thr2-Cys185(Gly12Val)) Protein, N-6*His-tagged

• Cat.No.: KRAS-454H
• Product Overview: Recombinant Human KRAS(AAH13572.1)(Thr2-Cys185(Gly12Val)) Protein, fused with a 6*His tag at the N-terminus, was produced in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 2-185 a.a.
• Form: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Molecular Mass: The recombinant Human KRAS Protein has a predicted molecular mass of 23.6kDa , it migrates with an apparent molecular mass of 25-30kDa under reducing conditions
• AA Sequence: Thr2-Cys185(Gly12Val)
• Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog [ Homo sapiens ]
• Official Symbol: KRAS
• Synonyms: KRAS; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog; KRAS2, v Ki ras2 Kirsten rat sarcoma 2 viral oncogene homolog; GTPase KRas; KRAS1; K-Ras 2; c-Ki-ras; oncogene KRAS2; K-ras p21 protein; c-Kirsten-ras protein; PR310 c-K-ras oncogene; transforming protein p21; cellular c-Ki-ras2 proto-oncogene; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog; NS; NS3; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
• Gene ID: 3845
• mRNA Refseq: NM_004985
• Protein Refseq: NP_004976
• MIM: 190070
• UniProt ID: P01116