Recombinant Human KRAS G12C Protein, His-tagged

• Cat.No.: KRAS-456H
• Product Overview: Recombinant Human KRAS G12C Protein(P01116-2) (Thr2-Cys185, with natural variant Gly 12 Cys) was expressed in E. coli with a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Thr2-Cys185, with natural variant Gly 12 Cys
• Tag: N-His
• Form: Lyophilized from sterile 25 mM Tris, 100 mM glycine, 10 % glycerol, pH 7.5.
• Bio-activity: The specific activity of KRAS was determined to be >2nmol/min/mg in a GTPase-Glo assay using GTP solution substrate.
• Molecular Mass: The recombinant human KRAS consists of 191 amino acids and predicts a molecular mass of 22 kDa.
• Purity: ≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C.

Gene Information

• Gene Name: KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog [ Homo sapiens ]
• Official Symbol: KRAS
• Synonyms: KRAS; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog; KRAS2, v Ki ras2 Kirsten rat sarcoma 2 viral oncogene homolog; GTPase KRas; KRAS1; K-Ras 2; c-Ki-ras; oncogene KRAS2; K-ras p21 protein; c-Kirsten-ras protein; PR310 c-K-ras oncogene; transforming protein p21; cellular c-Ki-ras2 proto-oncogene; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog; NS; NS3; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
• Gene ID: 3845
• mRNA Refseq: NM_004985
• Protein Refseq: NP_004976
• MIM: 190070
• UniProt ID: P01116