Recombinant Human KRAS, Isoform B, GDP-Loaded, N-His-tagged

• Cat.No.: KRAS-103H
• Product Overview: Recombinant human KRAS (KRAS proto-oncogene, GTPase), isoform b, encompassing amino acids 2-185. This construct contains an N-terminal His-tag (6×His). The recombinant protein was affinity purified and loaded with GDP. Unbound GDP was removed by spin column.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Protein Length: 2-186
• Description: This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.
• Form: Aqueous buffer solution
• Molecular Mass: 22 kDa
• Purity: ≥90%
• Storage Buffer: 20 mM HEPES, pH 7.4, 150 mM NaCl, and 1 mM DTT

Gene Information

• Gene Name: KRAS KRAS proto-oncogene, GTPase [ Homo sapiens (human) ]
• Official Symbol: KRAS
• Synonyms: KRAS; KRAS proto-oncogene, GTPase; NS; NS3; OES; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; K-Ras 2; 'C-K-RAS; c-Ki-ras; c-Ki-ras2; GTPase KRas; K-ras p21 protein; Kirsten rat sarcoma viral oncogene homolog; Kirsten rat sarcoma viral proto-oncogene; PR310 c-K-ras oncogene; c-Kirsten-ras protein; cellular c-Ki-ras2 proto-oncogene; cellular transforming proto-oncogene; oncogene KRAS2; proto-oncogene GTPase; transforming protein p21; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; EC 3.6.5.2
• Gene ID: 3845
• mRNA Refseq: NM_004985
• Protein Refseq: NP_004976
• MIM: 190070
• UniProt ID: P01116