Recombinant Human KRAS protein, His-Avi-tagged, Biotinylated

• Cat.No.: KRAS-2227H
• Product Overview: Recombinant Human KRAS protein(P01116-2)(Thr2-Cys185(G12D)) was expressed with a N-terminal polyhistidine tag followed by an Avi tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Specification

• Species: Human
• Source: E.coli
• Tag: Avi&His
• Protein Length: Thr2-Cys185(G12D)
• Tag: N-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile 50 mM Tris, pH8.0.
• Bio-activity: The specific activity of KRAS was determined to be >2nmol/min/mg in a GTPase-Glo assay using GTP solution substrate.
• Molecular Mass: The recombinant human KRAS consists of 206 amino acids and predicts a molecular mass of 23.78 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog [ Homo sapiens ]
• Official Symbol: KRAS
• Synonyms: KRAS; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog; KRAS2, v Ki ras2 Kirsten rat sarcoma 2 viral oncogene homolog; GTPase KRas; KRAS1; K-Ras 2; c-Ki-ras; oncogene KRAS2; K-ras p21 protein; c-Kirsten-ras protein; PR310 c-K-ras oncogene; transforming protein p21; cellular c-Ki-ras2 proto-oncogene; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog; NS; NS3; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
• Gene ID: 3845
• mRNA Refseq: NM_004985
• Protein Refseq: NP_004976
• MIM: 190070
• UniProt ID: P01116