Recombinant Human KRAS, His-tagged

• Cat.No.: KRAS-2569H
• Product Overview: Recombinant human KRAS protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 23.2 kDa (205aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: KRAS is a member of the small GTPase superfamily. This protein is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Under normal conditions, Ras family members influence cell growth and differentiation events in a subcellular membrane compartmentalization-based signaling system. Oncogenic Ras can deregulate processes that control both cell proliferation and apoptosis.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG HEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQI KRVKDSEDVP MVLVGNKCDL PSRTVDTKQA QDLARSYGIP FIETSAKTRQ GVDDAFYTLV REIRKHKEKM SKDGKKKKKK SKTKC
• Purity: > 90% by SDS – PAGE.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog [ Homo sapiens ]
• Synonyms: KRAS; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog; NS; NS3; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; GTPase KRas; K-Ras 2; c-Ki-ras; oncogene KRAS2; K-ras p21 protein; c-Kirsten-ras protein; PR310 c-K-ras oncogene; transforming protein p21; cellular c-Ki-ras2 proto-oncogene; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog; GTPase KRas, N-terminally processed
• Gene ID: 3845
• mRNA Refseq: NM_004985
• Protein Refseq: NP_004976
• MIM: 190070
• UniProt ID: P01116
• Chromosome Location: 12p12.1
• Pathway: Acute myeloid leukemia; Aldosterone-regulated sodium reabsorption; Axon guidance; B cell receptor signaling pathway; Bladder cancer; Chemokine signaling pathway; Chronic myeloid leukemia; Colorectal cancer; Dorso-ventral axis formation; Endometrial cancer; ErbB signaling pathway; Fc epsilon RI signaling pathway; Gap junction; Glioma; GnRH signaling pathway; Insulin signaling pathway; Long-term depression; Long-term potentiation; MAPK signaling pathway; Melanogenesis; Melanoma; Natural killer cell mediated cytotoxicity; Neurotrophin signaling pathway; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Progesterone-mediated oocyte; maturation; Prostate cancer; Regulation of actin cytoskeleton; Renal cell carcinoma; T cell receptor signaling pathway; Thyroid cancer; Tight junction; VEGF signaling pathway
• Function: GDP binding; GMP binding; GTP binding; GTPase activity; LRR domain binding; nucleotide binding; protein binding