Recombinant Human KPNA1 protein, His & T7-tagged

• Cat.No.: KPNA1-1313H
• Product Overview: Recombinant Human KPNA1 aa. (Leu239~Thr506 (Accession # P52294) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.3kDa.
• Protein length: Leu239~Thr506
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.9.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: KPNA1 karyopherin subunit alpha 1 [ Homo sapiens (human) ]
• Official Symbol: KPNA1
• Synonyms: KPNA1; karyopherin subunit alpha 1; RCH2; SRP1; IPOA5; NPI-1; importin subunit alpha-5; RAG cohort protein 2; SRP1-beta; importin alpha 5; importin subunit alpha-1; importin-alpha-S1; karyopherin alpha 1 (importin alpha 5); nucleoprotein interactor 1; recombination activating gene cohort 2
• Gene ID: 3836
• mRNA Refseq: NM_002264.3
• Protein Refseq: NP_002255.3
• UniProt ID: P52294