Recombinant Human KPNA1 293 Cell Lysate

• Cat.No.: KPNA1-4892HCL

Specification

• Description: Antigen standard for karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: KPNA1 karyopherin alpha 1 (importin alpha 5) [ Homo sapiens ]
• Official Symbol: KPNA1
• Synonyms: KPNA1; karyopherin alpha 1 (importin alpha 5); importin subunit alpha-1; IPOA5; NPI 1; RCH2; SRP1; SRP1-beta; importin alpha 5; importin-alpha-S1; RAG cohort protein 2; nucleoprotein interactor 1; karyopherin subunit alpha-1; recombination activating gene cohort 2; NPI-1
• Gene ID: 3836
• mRNA Refseq: NM_002264
• Protein Refseq: NP_002255
• MIM: 600686
• UniProt ID: P52294
• Chromosome Location: 3q21
• Pathway: Activation of DNA fragmentation factor, organism-specific biosystem; Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis induced DNA fragmentation, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; Class I PI3K signaling events mediated by Akt, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem
• Function: nuclear localization sequence binding; protein binding; protein transporter activity