Recombinant Human KNG1 protein, His-tagged

• Cat.No.: KNG1-6746H
• Product Overview: Recombinant Human KNG1 aa. (Ser390~Ser644 (Accession # P01042)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ser390~Ser644
• Description: This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. Bradykinin also functions as an antimicrobial peptide with antibacterial and antifungal activity. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.7kDa.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.4.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: KNG1 kininogen 1 [ Homo sapiens (human) ]
• Official Symbol: KNG1
• Synonyms: BK; BDK; KNG; HMWK; alpha-2-thiol proteinase inhibitor; bradykinin; fitzgerald factor; high molecular weight kininogen; williams-Fitzgerald-Flaujeac factor; kininogen-1
• Gene ID: 3827
• mRNA Refseq: NM_000893.3
• Protein Refseq: NP_000884.1
• UniProt ID: P01042