Recombinant Human KLRK1 protein(Phe78-Val216), His-tagged

• Cat.No.: KLRK1-8537H
• Product Overview: Recombinant Human NKG2D (P26718-1) (Phe78-Val216) was expressed in Insect Cells, fused with a polyhistide tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His
• Protein length: 78-216 a.a.
• Form: Lyophilized from sterile 20mM Tris, 300mM NaCl, 10%glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Immobilized human His-NKG2D (78-216) at 2 μg/ml (100 μl/well) can bind human ULBP1-hFc, The EC50 of human MICB-hFc is 8-50 ng/mL. 2. Immobilized human His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human ULBP1-hFc, The EC50 of human MICB-hFc is 15-60 ng/mL.
• Molecular Mass: The recombinant human NKG2D consists of 157 amino acids and has a calculated molecular mass of 18.4 kDa. As a result of glycosylation, the apparent molecular mass of human NKG2D is approximately 22.6 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: KLRK1 killer cell lectin-like receptor subfamily K, member 1 [ Homo sapiens ]
• Official Symbol: KLRK1
• Synonyms: KLRK1; killer cell lectin-like receptor subfamily K, member 1; D12S2489E, DNA segment on chromosome 12 (unique) 2489 expressed sequence; NKG2-D type II integral membrane protein; CD314; KLR; NKG2 D; NKG2D; NK cell receptor D; NKG2-D-activating NK receptor; NKG2-D; D12S2489E; FLJ17759; FLJ75772
• Gene ID: 22914
• mRNA Refseq: NM_007360
• Protein Refseq: NP_031386
• MIM: 611817
• UniProt ID: P26718